Chiusdino is a comune (municipality) in the Province of Siena in the Italian region Tuscany, located about 70 km south of Florence and about 30 km southwest of Siena. As of 31 December 2004, it had a population of 1,944 and an area of 142.1 km2.
The municipality of Chiusdino contains the frazione (subdivision) Ciciano.
Chiusdino borders the following municipalities: Casole d'Elsa, Monticiano, Montieri, Radicondoli, Roccastrada, Sovicille.
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